In Saint Sebastian, Agnolo Bronzino depicts the martyr in a dramatic pose. The figure is adorned with vibrant colors, primarily reds and greens, which contrast with the muted background. Sebastian's body is muscular and elongated, typical of Mannerist aesthetics.

The setting is minimal, focusing the viewer's attention on the saint's expression and the arrows piercing his flesh. The painting is executed in oil on panel, a common medium for the Renaissance period. Bronzino's technique showcases his skill in rendering textures, particularly in the depiction of skin and fabric. The use of light and shadow creates a sense of depth, enhancing the three-dimensionality of the figure.

The composition is balanced, with Sebastian positioned centrally, drawing the viewer's gaze. Agnolo Bronzino was a prominent figure of the Mannerist movement, known for his refined style and intricate details. This work reflects the artist's interest in the human form and emotional expression. Interestingly, Saint Sebastian was often commissioned for private devotion, making it a personal piece for its original owner.

Bronzino's ability to blend beauty with suffering is evident in this poignant portrayal of the saint.