A group of women in flowing garments surge forward from the right, led by a figure in a rose-colored dress who thrusts a spear toward a stag caught among a tangle of hounds at the center of the scene. Behind her, two nymphs in yellow and blue dresses follow in quick succession, one blowing a hunting horn and the other gripping her own spear, their faces animated with excitement and concentration. A fourth figure, partially obscured, raises her arm as if urging the chase onward, her golden hair catching the light.

The stag, eyes wide and legs splayed, tries to escape the snapping jaws of the dogs, which writhe and leap in a chaotic mass at its feet. The background opens onto a wooded glade, with trees arching overhead and dappled sunlight filtering through the leaves, casting warm highlights on the figures and animals. The palette is dominated by earthy browns and greens, punctuated by the vivid reds, yellows, and blues of the women's clothing, and the creamy white of the lead hound.

Loose, energetic brushstrokes give the scene a sense of movement and immediacy, especially in the fur of the animals and the swirling drapery of the nymphs. The composition sweeps from right to left, with the figures and animals forming a diagonal line that drives the action across the panel. Paint is applied with visible, textured strokes, particularly in the foreground where the dogs and undergrowth merge in a flurry of color and line. Diana and her Nymphs Hunting is a mythological painting by Peter Paul Rubens, created in the 1630s.

A spear, a stag, and the act of hunting are all central elements depicted in the work.